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Kosugi, T. and Inouye, K. (1989). Negative chemotaxis to ammonia and other weak bases by migrating slugs of the cellular slime moulds. J. gen. microbiol. 135, 1589-1598.

Movies in QuickTime (mac, unix) and avi (wondows) formats --- (size: 230-500 kb)
Movie QuickTime
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avi
Figure 6. Repeated turns by a Dictyostelium discoideum slug induced by alternating the direction of a gradient of ethylamine. The anterior region of the slug extends in the air from the agar block which is seen in the lower right corner. The gas source is a glass capillary containing 3%(w/v) ethylamine solution.
Movie: 31 min compressed to 7 sec, field width: approx. 0.5 mm.
Movie QuickTime
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avi
Figure 7. Repeated turns of a Polysphondylium violaceum slug induced by alternating the direction of a gradient of ammonia. The vertical dark line in the middle is the edge of the agar block which lies on the right-hand side. The slug had been migrating on the agar block, and when it passed the edge of the agar block, it became supported only by its stalk which had been continuously made by the slug itself. In the latter half of the movie, the slug is completely in the air, indicating that ammonia directly acts on the slug, not via the agar substratum. The glass capillary contains 10%(v/v) ammonia gas.
Movie: 1 hour 50 min compressed to 4.5 sec, field width: approx. 0.9 mm.
Movie QuickTime
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avi
Figure 8. Quantitation of chemotactic responses of migrating slugs to ammonia. A micropipette filled with NH3 solution was introduced at t = 0 min and removed at t = 6 min. The angles between the directions of the slugs at t = 0 min and at t = 12 min (movie) were measured and related to the NH3 gradients at t = 6 min (graph on the right).
Movie: 12 min compressed to 3 sec, field width: approx. 0.6 mm.
angle vs ammonia gradient graph

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