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Fig.2
Movie of fluorescence images of an Ax2 cell with an extending protrusion. Cells had been stained with RH-795 10 minutes before quinine application. This dye binds to contractile vacuole membranes with high affinity. Note the highly fluorescent patch in the leading edge of the protrusion. 0.62 sec/frame, field width 30 micrometres. |
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Fig.4
Movie showing concanavalin A-tagged beads attached to the cell surface during elongation of protrusion. Fluorescence is shown in red and Nomarski images in green. 0.55 sec/frame, field width 31 micrometres. |
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Fig.6C
Movie of fluorescence images of an mhc-null cell showing expulsion of a contractile vacuole. The cell had been stained with RH-795 10 minutes before quinine application. A patch of fluorescence persists on the cell membrane at the site of expulsion of one of the contractile vacuoles. 0.72 sec/frame, field width 17 micrometres. |
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Fig.7A
Dynamics of GFP-myosin II during the formation of a protrusion in the presence of quinine. 3.2 sec/frame, field width 31 micrometres. |
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Fig.7B
Another cell showing a travelling band of GFP-myosin II on the protrusion towards its tip. 1.3 sec/frame, field width 44 micrometres. |
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Fig.10A
Movie of a GFP-ABD cell exposed to quinine. 2.2 sec/frame, field width 39 micrometres. |
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Fig.10B
Movie of a GFP-ABD cell exposed to quinine. 1.4 sec/frame, field width 63 micrometres. |
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Fig.11
Dynamics of GFP-myosin II during bleb formation in the presence of quinine and cytochalasin A. 0.70 sec/frame, field width 17 micrometres. |
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Fig.12
Movie of a locomoting slug cell in the absence of quinine. Slugs migrating on agar were dissociated into single cells in phosphate buffer by repeated passage through a 25G needle, and plated on a coverslip in such a way that the buffer formed a thin film of a depth just enough to cover the cells. 1.0 sec/frame, field width 17 micrometres. |