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Microscopes: 
(A1a) Nikon dissecting SMZ-10, transmitted light (no angle deviation)
(A1b) Nikon dissecting SMZ-10, incident light
(A2a) Nikon inverted TMD, transmitted light
(A2b) Nikon inverted TMD, epi-fluorescence, halogen lamp, heat/UV absorption filters, ex/em: 510/520-560 nm
(A2c) Nikon inverted TMD, epi-fluorescence, LED, heat/UV absorption filters, ex/em: 510/520-560 nm
(A3)  Pika travelling PRM-2, fibre optic incident lighting
(B)   Zeiss LSM410 confocal
(C1)  Without microscope (camera on tripod, 50 mm Olympus macro lens)
(C2)  Without microscope (lab-made camera holder with variable angle, 100 mm Olympus macro lens)

Camera etc (A1-A3, C1-C2):
   Camera: Tokyo Denshi CCD video camera CS8310, 2/3 inches
   Computer: Macintosh PM7600, Scion AG-5 board; Macintosh G3, Scion LG-5 board
   Software: NIH Image, (mostly video-rate) averaging
   Lighting: mostly intermittent illumination

Camera etc (A2c):
   Camera: Bitran CCCD BU-40L
   Computer: Dell Precision 360
   Software: ImageJ, (mostly video-rate) averaging
   Lighting: blue LED, intermittent illumination (500 msec pulses)



Temperature: ca. 22 degrees celcius unless otherwise indicated)

From growth to aggregation and slug formation

c01 D. rosarium (A1a)
    (Dr_8min) 250x150, 63 frames, 25 um/pixel, 6.25 mm/span, 8 min/frame, duration 8 h 16 min
    (original) 640x480, 300 frames, 12.5 um/pixel, 8.0 mm/span, 2 min/frame, duration 10 h

c02 P. violaceum (A1a)
  c02-1 (Pv_8min) 250x150, 63 frames, 25 um/pixel, 6.25 mm/span, 8 min/frame, duration 8 h 16 min
  c02-2(Pv_1min) 240x160, 180 frames, 12.5 um/pixel, 3.0 mm/span, 1 min/frame, duration 4 h 26 min
    (original) 640x480, 500 frames, 12.5 um/pixel, 8.0 mm/span, 10 and 20 sec/frame, duration 8 h 20 min

c03 D. mucoroides (A1a)
    (Dm_8min) 250x150, 75 frames, 25 um/pixel, 6.25 mm/span, 8 min/frame, duration 10 h
    (original) 640x480, 300 frames, 12.5 um/pixel, 8.0 mm/span, 2 min/frame, duration 10 h

c04 D. discoideum (C1)
    (V12M2_plate) 320x240, 71 frames, 238 um/pixel, 76.2 mm/span, 1 h/frame, duration 70 h
    (original) 640x480, 718 frames, 119 um/pixel, 80.9 mm/span, 2 min/frame, duration 70 h

c05 P. pallidum (C2)
    (Pp_plate) 240x160, 60 frames, 38.0 um/pixel, 9.12 mm/span, 16 min/frame, duration 15 h 44 min
    (original) 640x480, 479 frames, 19.0 um/pixel, 12.16 mm/span, 2 min/frame, duration 16 h

Growth phase

v01 D. rosarium (A1a)
    (Dr_veg_wide) 200x180, 91 frames, 2.60 um/pixel, field span 520 um, 1 min/frame, duration 1 h 30 min
    (original) 640x480, 271 frames, 20 sec/frame

v02 D. discoideum cell division (A1a)
    (M2_division_3) 160x160, 82 frames, 0.318 um/pixel, scale bar 10 um, 10 sec/frame, 13 min 40 sec
    (original) 5 sec/frame

v03 D. discoideum feeding (A1a)
    (M2_feeding_3) 172x136, 101 frames, 0.318 um/pixel, scale bar 10 um, 5 sec/frame, 8 min 25 sec
    (original) 439x445, 260 frames, no compression
    
v04 D. discoideum phagocytosis (B)
    (talBCg_phagocytosis) 160x144, 52 frames, 0.138 um/pixel, scale bar 5 um, 5 sec/frame, 4 min 15 sec, green: gfp fluorescence, grey: Nomarski

v05 D. discoideum pinocytosis (B)
    (talBCg_pinocytosis) 160x144, 45 frames, 0.278 um/pixel, scale bar 10 um, 5 sec/frame, 3 min 40 sec, green: gfp fluorescence, red: rhodamine fluorescence

v06 Germination (A1a)
  v06-1 (Ar_germ_1_small) 180x120, 140 frames, 0.637 um/pixel, 115 um/span, scale bar 10 um, 10 sec/frame, 23 min 10 sec
  v06-2 (Ar_germ_2) 330x225, 112 frames, 0.425 um/pixel, 140 um/span, 10 sec/frame, 18 min 30 sec
    (original) 465x478, 600 frames, 0.318 um/pixel, 10 sec/frame
    
v07 Feeding (A1a)
    (Ar_feeding_4sec) 128x165, 86 frames, 0.425 um/pixel,  scale bar 10 um, 10 sec/frame, 14 min 10 sec

v08 D. mucoroides 7 (A2a)
    (Dm_feeding) 144x204, 67 frames, 0.318 um/pixel, 45.8 um/span, 5 sec/frame, duration 5 min 30 sec
    (original) 640x480, 600 frames, 0.318 um/pixel

v09 D. mucoroides 7 (A2a)
    (Dm_longbacterium) 200x120, 175 frames, 0.318 um/pixel, 63.6 um/span, 20 sec/frame, duration 58 min
    (original) -

Aggregation

a01 P. violaceum aggregation stream formation (A1a)
    (Pv_agg_4_s) 200x120, 50 frames, 6.43 um/pixel, 1.3 mm/span, 1 min/frame, duration 50 min
    (original) 600x400, 587 frames, 3.22 um/pixel, 15 sec/frame, duration 2 h 26 min

a02 D. discoideum Ax2 spiral aggregates (A1a)
  a02-1 (Ax2_spiral) 250x175, 64 frames, 37.5 um/pixel, 9.4 mm/span, 2 min/frame, duration 2 h 7 min
  a02-2 (Ax2_spiral_diff) 250x175, 36 frames, 37.5 um/pixel, 9.4 mm/span, 60 sec/frame, duration 35 min
    (original) 640x480, 600 frames, 18.75 um/pixel, 12.0 mm/span, 60 sec/frame, duration 10 hours

a03 D. discoideum Ax2 (A1a)
  a03-1,2 (Ax2_stream_40) 300x105, 51 frames, 25.0 um/pixel, 7.5 mm/span, 40 sec/frame, diff 40 sec, duration 39 min 20 sec
    (original) 500x300, 600 frames, 18.75 um/pixel, 9.4 mm/span, 20 sec/frame, duration 200 min

a04 D. discoideum Ax2 (in the same plate as above) (A1a)
    (Ax2_stream_30) 180x100, 80 frames, 18.75 um/pixel, 3.4 mm/span, 30 sec/frame, diff 60 sec, duration 40 min
    (original) 640x346, 300 frames, 18.75 um/pixel, 12.0 mm/span, 30 sec/frame, duration 150 min

    // Typical oscillation data of this cell population
          period 197 sec
	  wave length 170 um
	  wave propagation speed 0.9 um/sec = 52 um/min //

a05 D. discoideum Ax2 HG1664 (A1a)
    (Ax2_rings_Merged) 76x136, 108 frames, 12.5 um/pixel, 0.95 mm/span, 80 sec/frame, diff 40 sec, duration 143 min
    (original) 512x235, 282 frames, 12.5 um/pixel, 40 sec/frame

a06 D. discoideum Ax2  HG1664 (in the same plate as above) (A1a)
    (Ax2_largering_Merged) 54x92, 149 frames, 12.5 um/pixel x 2, 1.35 mm/span, 2 min/frame, diff 30 sec, duration 296 min
    (original) 484x299, 600 frames, 12.5 um/pixel, 30 sec/frame

a07 D. lacteum (A3)
    (Dl_slug_s) 180x108, 81 frames, 3.39 um/pixel x 2, 1.22 mm/span, 10 min/frame, duration 13 h 20 min
    (original) 400x480, 303 frames, 3.39 um/pixel, 1 min/frame, 5 min/frame

a08 D. polycephalum (A1a)
    (Dpoly_agg_1_s) 200x200, 61 frames, 5.05 um/pixel x 3/2, 1.515 mm/span, 4 min/frame, duration 4 h
    (original) 680x480, 357 frames, 5.05 um/pixel, 1 min/frame

Slug migration and fruiting body formation

s01 P. violaceum slug migration (A3)
    (Pv_slug_s) 220x120, 44 frames, 3.72 um/pixel x 2, 1637 um/field width, 2 min/frame, duration 88 min
    (original) 640x480, 44 frames, 3.72 um/pixel, 1 min/frame

s02 D. discoideum slug migration (A3)
    (Dd_slug_1) 280x80, 76 frames, 3.39 um/pixel x 2, 1900 um/field width, 1 min/frame, duration 75 min
    (original) 640x480, 91 frames, 3.39 um/pixel, 1 min/frame

s03 D. discoideum (chimeric slug of Ax2 and Ax2 transformed with actin15-GFP) (A2b)
    (a15GFP_mix) 290x93, 65 frames, 2.6 um/pixel, 753 um/field width, 20 sec/frame, duration 21 min 40 sec
    (original) 636x240, 240 frames, 1.3 um/pixel, 826 um/field width, 10 sec/frame, duration 40 min

s04 D. discoideum fruiting (A3)
    (Dd_cul_1s) 120x240, 58 frames, 3.39 um/pixel x 2, 814 um/field width, 8 min/frame, duration 456 min
    (original) 640x480, 581 frames, 3.39 um/pixel, 1 min/frame

s05 Dictyostelium purpureum culmination (A3)
    (Dp_cul_8min_s) 190x170, 90 frames, 3.75 um/pixel x 2, 1414 um/field width, 8 min/frame, duration 12 hours

s06 Dictyostelium purpureum sorus (A1a)
    (Dp_sporulation_1) 60x100, 75 frames, 12.5 um/pixel, 750 um/field width, 4 min/frame, duration 5 hours
    (original) 388x464, 335 frames, 2 min/frame, duration 11 h 10 min

s07 Acrasis rosea culmination (A3)
    (Ar_fruiting_2) 252x135, 65 frames, 3.39 um/pixel x 4/3, 1140 um/field width, 1 min/frame, duration 64 min
    (original) 602x386, 300 frames, 3.39 um/pixel

s08 D. discoideum slug migration (A3)
    (Dd_slug_2) 400x100, 77 frames, 6.78 um/pixel, 2712 um/field width, 1 min/frame, duration 76 min
    (original) 640x240, 131 frames, 6.78 um/pixel, 1 min/frame

s09 P. violaceum slug migration (A3)
    (Pv_slug_2_s) 300x116, 74 frames, 6.78 um/pixel x 2, 4068 um/field width, 2 min/frame, duration 146 min
    (original) 640x480, 268 frames, 6.78 um/pixel, 1 min/frame

s10 P. pallidum fruiting (A3)
    (Pp_cul_1_s) 134x208, 50 frames, 6.78 um/pixel x 4/3, 1211 um/field width, 5 min/frame, duration 4 h 5 min
    (original) 393x447, 951 frames, 6.78 um/pixel, 30 sec/frame

s11 D. polycephalum slug formation (A3)
    (Dpoly_slug) 192x144, 53 frames, 6.78 um/pixel x 3, 3905 um/field width, 8 min/frame, duration 7 h
    (original) 640x480, 689 frames, 6.78 um/pixel, 1 min/frame, 4 min/frame, ca. 25 degrees celcius

s12 D. giganteum slug showing phototaxis (A1a)
    (Dg_phototaxis_2_ss) 192x160, 85 frames, 18.75 um/pixel x 2, 7200 um/field width, 4 min/frame, duration 5 h 36 min
    (original) 640x480, 446 frames, 18.75 um/pixel, 1 min/frame

s13 D. polycephalum slugs (C2)
    (Dpoly_slug_3_s) 126x220, 117 frames, 33.4 um/pixel x 2, 8,417 um/field width, 20 min/frame, duration 38 h 40 min
    (original) 640x480, 468 frames, 33.4 um/pixel, 5 min/frame, ca. 25 degrees celcius

s14 D. aureo-stipes culmination (C2)
    (Ddel_1) 270x195, 99 frames, 12.52 um/pixel, 3,380 um/field width, 8 min/frame, duration 13 h 4 min
    (original) 600x400, 600 frames, 7.04 um/pixel, 2 min/frame

s15 D. aureo-stipes branching (C2)
    (Ddel_2) 200x240, 85 frames, 7.04 um/pixel, 1,408 um/field width, 10 min/frame, duration 14 h 0 min
    (original) 640x480, 251 frames, 7.04 um/pixel, 5 min/frame

s16 D. polycephalum culmination (A1b)
    (Dpoly_cul_1) 100x144, 144 frames, 3.13 um/pixel, 313 um/field width, 4 min/frame, duration 9 h 32 min
    (original) 100x144, 312 frames at 1 min/frame and 217 frames at 4 min/frame, 3.13 um/pixel

s17 D. polycephalum culmination (A2a)
    (Dpoly_cul_3) 150x270, 71 frames, 1.73 um/pixel, 260 um/field width, 8 min/frame, duration 9 h 20 min
    (original) 640x480, 297 frames, 1.30 um/pixel, 2 min/frame

s18 D. minutum aggregation and culmination (C2)
    (Dmin_1) 340x200, 66 frames, 9.32 um/pixel, 3,168 um/field width, 12 min/frame, duration 13 h 0 min
    (original) 640x480, 198 frames, 6.21 um/pixel, 4 min/frame

s19 D. discoideum slug (A2b)
    (Dd_gfp_slug_s) 360x200, 60 frames, 1.68 um/pixel, 600 um/field width, 10 sec/frame, duration 10 min
    (original) 772x580, 60 frames, 0.84 um/pixel, 10 sec/frame

s20 D. rosarium slug (C2)
    (Dr_slug_1) 480x200, 180 frames, 7.75 um/pixel, 3,721 um/field width, 2 min/frame, duration 6 h
    (original) 560x280, 434 frames, 7.75 um/pixel, 1 min/frame

Macrocyst formation

m01 D. mucoroides aggregation and macrocyst formation (A2a)
    (Dm_MC_s) 178x119, 151 frames, 3.22 um/pixel x 2, 1146 um/field width, 4 min/frame, duration 600 min
    (original) 640x480, 410 frames, 3.22 um/pixel, 2 min/frame

m02 D. mucoroides macrocyst formation early stage (A2a)
    (Dm_MC_early) 160x144, 115 frames, 0.318 um/pixel x 2, 101.8 um/field width, 2 min/frame, duration 3 h 48 min
    (original) 482x337, 504 frames and 401x338, 506 frames, 0.318 um/pixel, 1 min/frame

m03 D. mucoroides macrocyst formation (A2a)
    (Dm_MC_mid) 160x144, 26 frames, montage only, 0.318 um/pixel x 2, 101.8 um/field width, 1 h/frame, duration 26 h
    (original) 401x338, 146 frames, 0.318 um/pixel, 5 min/frame and 10 min/frame and 20 min/frame


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